@tee-doc
25I'm ogunbiyi toluwalope, (a.k.a tee-doc), a veterinarian ,an animal lover and also a public health personnel .
steemit.com/@tee-docVOTING POWER100.00%
DOWNVOTE POWER100.00%
RESOURCE CREDITS100.00%
REPUTATION PROGRESS0.00%
Net Worth
0.018USD
STEEM
0.003STEEM
SBD
0.020SBD
Effective Power
5.001SP
├── Own SP
0.143SP
└── Incoming DelegationsDeleg
+4.858SP
Detailed Balance
| STEEM | ||
| balance | 0.001STEEM | STEEM |
| market_balance | 0.000STEEM | STEEM |
| savings_balance | 0.002STEEM | STEEM |
| reward_steem_balance | 0.000STEEM | STEEM |
| STEEM POWER | ||
| Own SP | 0.143SP | SP |
| Delegated Out | 0.000SP | SP |
| Delegation In | 4.858SP | SP |
| Effective Power | 5.001SP | SP |
| Reward SP (pending) | 0.000SP | SP |
| SBD | ||
| sbd_balance | 0.000SBD | SBD |
| sbd_conversions | 0.000SBD | SBD |
| sbd_market_balance | 0.000SBD | SBD |
| savings_sbd_balance | 0.020SBD | SBD |
| reward_sbd_balance | 0.000SBD | SBD |
{
"balance": "0.001 STEEM",
"savings_balance": "0.002 STEEM",
"reward_steem_balance": "0.000 STEEM",
"vesting_shares": "232.466187 VESTS",
"delegated_vesting_shares": "0.000000 VESTS",
"received_vesting_shares": "7911.193619 VESTS",
"sbd_balance": "0.000 SBD",
"savings_sbd_balance": "0.020 SBD",
"reward_sbd_balance": "0.000 SBD",
"conversions": []
}Account Info
| name | tee-doc |
| id | 918045 |
| rank | 419,189 |
| reputation | 311043414 |
| created | 2018-04-04T09:33:48 |
| recovery_account | steem |
| proxy | None |
| post_count | 15 |
| comment_count | 0 |
| lifetime_vote_count | 0 |
| witnesses_voted_for | 1 |
| last_post | 2018-11-18T00:15:45 |
| last_root_post | 2018-06-18T16:43:36 |
| last_vote_time | 2018-11-18T00:11:33 |
| proxied_vsf_votes | 0, 0, 0, 0 |
| can_vote | 1 |
| voting_power | 0 |
| delayed_votes | 0 |
| balance | 0.001 STEEM |
| savings_balance | 0.002 STEEM |
| sbd_balance | 0.000 SBD |
| savings_sbd_balance | 0.020 SBD |
| vesting_shares | 232.466187 VESTS |
| delegated_vesting_shares | 0.000000 VESTS |
| received_vesting_shares | 7911.193619 VESTS |
| reward_vesting_balance | 0.000000 VESTS |
| vesting_balance | 0.000 STEEM |
| vesting_withdraw_rate | 0.000000 VESTS |
| next_vesting_withdrawal | 1969-12-31T23:59:59 |
| withdrawn | 0 |
| to_withdraw | 0 |
| withdraw_routes | 0 |
| savings_withdraw_requests | 0 |
| last_account_recovery | 1970-01-01T00:00:00 |
| reset_account | null |
| last_owner_update | 1970-01-01T00:00:00 |
| last_account_update | 2018-04-09T10:45:09 |
| mined | No |
| sbd_seconds | 8,520 |
| sbd_last_interest_payment | 2018-06-28T22:19:39 |
| savings_sbd_last_interest_payment | 2018-06-28T22:27:57 |
{
"id": 918045,
"name": "tee-doc",
"owner": {
"weight_threshold": 1,
"account_auths": [],
"key_auths": [
[
"STM5Q3nsqqHmroWUjPbWCzKe1a7ueUm4zgnJ7c5eF6sq3mKuhJbkY",
1
]
]
},
"active": {
"weight_threshold": 1,
"account_auths": [],
"key_auths": [
[
"STM5uj4NKw3DwjURLtYHLPRFJxxf2freWHDCNn1kqLkLuiPGmeD42",
1
]
]
},
"posting": {
"weight_threshold": 1,
"account_auths": [
[
"dlive.app",
1
]
],
"key_auths": [
[
"STM79kNVuvNdLciXt1FwFqtfDuDsKYaoER53PUumZR692RdNtPws9",
1
]
]
},
"memo_key": "STM5ptoJFmq4w9dNPTjNGmsG8KpinK9C3QuTYAGMyzd8M9eZttQam",
"json_metadata": "{\"profile\":{\"name\":\"Tee-doc\",\"about\":\"I'm ogunbiyi toluwalope, (a.k.a tee-doc), a veterinarian ,an animal lover and also a public health personnel .\",\"location\":\"Ogun state Nigeria \"}}",
"posting_json_metadata": "{\"profile\":{\"name\":\"Tee-doc\",\"about\":\"I'm ogunbiyi toluwalope, (a.k.a tee-doc), a veterinarian ,an animal lover and also a public health personnel .\",\"location\":\"Ogun state Nigeria \"}}",
"proxy": "",
"last_owner_update": "1970-01-01T00:00:00",
"last_account_update": "2018-04-09T10:45:09",
"created": "2018-04-04T09:33:48",
"mined": false,
"recovery_account": "steem",
"last_account_recovery": "1970-01-01T00:00:00",
"reset_account": "null",
"comment_count": 0,
"lifetime_vote_count": 0,
"post_count": 15,
"can_vote": true,
"voting_manabar": {
"current_mana": "8143659806",
"last_update_time": 1779088548
},
"downvote_manabar": {
"current_mana": 2035914951,
"last_update_time": 1779088548
},
"voting_power": 0,
"balance": "0.001 STEEM",
"savings_balance": "0.002 STEEM",
"sbd_balance": "0.000 SBD",
"sbd_seconds": "8520",
"sbd_seconds_last_update": "2018-06-28T22:26:45",
"sbd_last_interest_payment": "2018-06-28T22:19:39",
"savings_sbd_balance": "0.020 SBD",
"savings_sbd_seconds": "0",
"savings_sbd_seconds_last_update": "2018-06-28T22:33:09",
"savings_sbd_last_interest_payment": "2018-06-28T22:27:57",
"savings_withdraw_requests": 0,
"reward_sbd_balance": "0.000 SBD",
"reward_steem_balance": "0.000 STEEM",
"reward_vesting_balance": "0.000000 VESTS",
"reward_vesting_steem": "0.000 STEEM",
"vesting_shares": "232.466187 VESTS",
"delegated_vesting_shares": "0.000000 VESTS",
"received_vesting_shares": "7911.193619 VESTS",
"vesting_withdraw_rate": "0.000000 VESTS",
"next_vesting_withdrawal": "1969-12-31T23:59:59",
"withdrawn": 0,
"to_withdraw": 0,
"withdraw_routes": 0,
"curation_rewards": 2,
"posting_rewards": 23,
"proxied_vsf_votes": [
0,
0,
0,
0
],
"witnesses_voted_for": 1,
"last_post": "2018-11-18T00:15:45",
"last_root_post": "2018-06-18T16:43:36",
"last_vote_time": "2018-11-18T00:11:33",
"post_bandwidth": 0,
"pending_claimed_accounts": 0,
"vesting_balance": "0.000 STEEM",
"reputation": 311043414,
"transfer_history": [],
"market_history": [],
"post_history": [],
"vote_history": [],
"other_history": [],
"witness_votes": [
"scottcbusiness"
],
"tags_usage": [],
"guest_bloggers": [],
"rank": 419189
}Withdraw Routes
| Incoming | Outgoing |
|---|---|
Empty | Empty |
{
"incoming": [],
"outgoing": []
}From Date
To Date
2026/05/18 07:15:48
2026/05/18 07:15:48
| delegator | steem |
| delegatee | tee-doc |
| vesting shares | 7911.193619 VESTS |
| Transaction Info | Block #106151826/Trx 6df9566b247c03bd0b2c70b1d40601c49b99239e |
View Raw JSON Data
{
"trx_id": "6df9566b247c03bd0b2c70b1d40601c49b99239e",
"block": 106151826,
"trx_in_block": 6,
"op_in_trx": 0,
"virtual_op": 0,
"timestamp": "2026-05-18T07:15:48",
"op": [
"delegate_vesting_shares",
{
"delegator": "steem",
"delegatee": "tee-doc",
"vesting_shares": "7911.193619 VESTS"
}
]
}2026/05/13 08:20:15
2026/05/13 08:20:15
| delegator | steem |
| delegatee | tee-doc |
| vesting shares | 5198.983214 VESTS |
| Transaction Info | Block #106009828/Trx b42f602623cb79be66b02f69f34cbbeb4bab8fda |
View Raw JSON Data
{
"trx_id": "b42f602623cb79be66b02f69f34cbbeb4bab8fda",
"block": 106009828,
"trx_in_block": 4,
"op_in_trx": 0,
"virtual_op": 0,
"timestamp": "2026-05-13T08:20:15",
"op": [
"delegate_vesting_shares",
{
"delegator": "steem",
"delegatee": "tee-doc",
"vesting_shares": "5198.983214 VESTS"
}
]
}2026/04/26 06:26:18
2026/04/26 06:26:18
| delegator | steem |
| delegatee | tee-doc |
| vesting shares | 7923.709375 VESTS |
| Transaction Info | Block #105519284/Trx 52119a15b819bab009e25b34cbcf0e68eee826cf |
View Raw JSON Data
{
"trx_id": "52119a15b819bab009e25b34cbcf0e68eee826cf",
"block": 105519284,
"trx_in_block": 0,
"op_in_trx": 0,
"virtual_op": 0,
"timestamp": "2026-04-26T06:26:18",
"op": [
"delegate_vesting_shares",
{
"delegator": "steem",
"delegatee": "tee-doc",
"vesting_shares": "7923.709375 VESTS"
}
]
}2026/01/24 02:40:42
2026/01/24 02:40:42
| delegator | steem |
| delegatee | tee-doc |
| vesting shares | 5240.530033 VESTS |
| Transaction Info | Block #102874416/Trx 1f68d718f5e328fdd7a9152587bf222008706e1c |
View Raw JSON Data
{
"trx_id": "1f68d718f5e328fdd7a9152587bf222008706e1c",
"block": 102874416,
"trx_in_block": 1,
"op_in_trx": 0,
"virtual_op": 0,
"timestamp": "2026-01-24T02:40:42",
"op": [
"delegate_vesting_shares",
{
"delegator": "steem",
"delegatee": "tee-doc",
"vesting_shares": "5240.530033 VESTS"
}
]
}2024/12/17 21:49:30
2024/12/17 21:49:30
| delegator | steem |
| delegatee | tee-doc |
| vesting shares | 5404.749230 VESTS |
| Transaction Info | Block #91320612/Trx 67555b95da1c497b34c6a081ae97d0d3f4f62e21 |
View Raw JSON Data
{
"trx_id": "67555b95da1c497b34c6a081ae97d0d3f4f62e21",
"block": 91320612,
"trx_in_block": 4,
"op_in_trx": 0,
"virtual_op": 0,
"timestamp": "2024-12-17T21:49:30",
"op": [
"delegate_vesting_shares",
{
"delegator": "steem",
"delegatee": "tee-doc",
"vesting_shares": "5404.749230 VESTS"
}
]
}2023/11/14 13:28:42
2023/11/14 13:28:42
| delegator | steem |
| delegatee | tee-doc |
| vesting shares | 5573.882762 VESTS |
| Transaction Info | Block #79874716/Trx 353e1e03d355bb6d63ad43bbdb990a65d2b99cdd |
View Raw JSON Data
{
"trx_id": "353e1e03d355bb6d63ad43bbdb990a65d2b99cdd",
"block": 79874716,
"trx_in_block": 0,
"op_in_trx": 0,
"virtual_op": 0,
"timestamp": "2023-11-14T13:28:42",
"op": [
"delegate_vesting_shares",
{
"delegator": "steem",
"delegatee": "tee-doc",
"vesting_shares": "5573.882762 VESTS"
}
]
}2023/09/22 11:32:57
2023/09/22 11:32:57
| delegator | steem |
| delegatee | tee-doc |
| vesting shares | 8510.791548 VESTS |
| Transaction Info | Block #78364249/Trx b0124c2e0bad61e6a11533ce6e7e1e2f78270f0c |
View Raw JSON Data
{
"trx_id": "b0124c2e0bad61e6a11533ce6e7e1e2f78270f0c",
"block": 78364249,
"trx_in_block": 2,
"op_in_trx": 0,
"virtual_op": 0,
"timestamp": "2023-09-22T11:32:57",
"op": [
"delegate_vesting_shares",
{
"delegator": "steem",
"delegatee": "tee-doc",
"vesting_shares": "8510.791548 VESTS"
}
]
}2022/11/03 18:52:12
2022/11/03 18:52:12
| delegator | steem |
| delegatee | tee-doc |
| vesting shares | 8732.842986 VESTS |
| Transaction Info | Block #69121820/Trx fc36bd013f816df5c64b36dfa49cadc9fa3e8309 |
View Raw JSON Data
{
"trx_id": "fc36bd013f816df5c64b36dfa49cadc9fa3e8309",
"block": 69121820,
"trx_in_block": 0,
"op_in_trx": 0,
"virtual_op": 0,
"timestamp": "2022-11-03T18:52:12",
"op": [
"delegate_vesting_shares",
{
"delegator": "steem",
"delegatee": "tee-doc",
"vesting_shares": "8732.842986 VESTS"
}
]
}2022/01/17 23:58:06
2022/01/17 23:58:06
| delegator | steem |
| delegatee | tee-doc |
| vesting shares | 8952.950587 VESTS |
| Transaction Info | Block #60824952/Trx a212e315160bde550b167dcac37f2f26cecf8bc8 |
View Raw JSON Data
{
"trx_id": "a212e315160bde550b167dcac37f2f26cecf8bc8",
"block": 60824952,
"trx_in_block": 26,
"op_in_trx": 0,
"virtual_op": 0,
"timestamp": "2022-01-17T23:58:06",
"op": [
"delegate_vesting_shares",
{
"delegator": "steem",
"delegatee": "tee-doc",
"vesting_shares": "8952.950587 VESTS"
}
]
}2021/06/14 07:06:30
2021/06/14 07:06:30
| delegator | steem |
| delegatee | tee-doc |
| vesting shares | 9137.144875 VESTS |
| Transaction Info | Block #54615220/Trx 78bf59b5a9a0fed80fe32930f6e33da7ed5a9166 |
View Raw JSON Data
{
"trx_id": "78bf59b5a9a0fed80fe32930f6e33da7ed5a9166",
"block": 54615220,
"trx_in_block": 2,
"op_in_trx": 0,
"virtual_op": 0,
"timestamp": "2021-06-14T07:06:30",
"op": [
"delegate_vesting_shares",
{
"delegator": "steem",
"delegatee": "tee-doc",
"vesting_shares": "9137.144875 VESTS"
}
]
}2020/12/11 17:17:57
2020/12/11 17:17:57
| delegator | steem |
| delegatee | tee-doc |
| vesting shares | 9324.566849 VESTS |
| Transaction Info | Block #49362457/Trx 3af5b5bd587d72ee74fee20137623755729132a3 |
View Raw JSON Data
{
"trx_id": "3af5b5bd587d72ee74fee20137623755729132a3",
"block": 49362457,
"trx_in_block": 3,
"op_in_trx": 0,
"virtual_op": 0,
"timestamp": "2020-12-11T17:17:57",
"op": [
"delegate_vesting_shares",
{
"delegator": "steem",
"delegatee": "tee-doc",
"vesting_shares": "9324.566849 VESTS"
}
]
}2020/12/06 10:53:15
2020/12/06 10:53:15
| delegator | steem |
| delegatee | tee-doc |
| vesting shares | 1912.543513 VESTS |
| Transaction Info | Block #49213968/Trx 1ffc776f026b3150d9766991e220eb464717e425 |
View Raw JSON Data
{
"trx_id": "1ffc776f026b3150d9766991e220eb464717e425",
"block": 49213968,
"trx_in_block": 13,
"op_in_trx": 0,
"virtual_op": 0,
"timestamp": "2020-12-06T10:53:15",
"op": [
"delegate_vesting_shares",
{
"delegator": "steem",
"delegatee": "tee-doc",
"vesting_shares": "1912.543513 VESTS"
}
]
}2020/12/05 20:55:51
2020/12/05 20:55:51
| delegator | steem |
| delegatee | tee-doc |
| vesting shares | 9330.774703 VESTS |
| Transaction Info | Block #49197541/Trx d599ca465657fe54120d3b5a22f98bf11f6439fe |
View Raw JSON Data
{
"trx_id": "d599ca465657fe54120d3b5a22f98bf11f6439fe",
"block": 49197541,
"trx_in_block": 8,
"op_in_trx": 0,
"virtual_op": 0,
"timestamp": "2020-12-05T20:55:51",
"op": [
"delegate_vesting_shares",
{
"delegator": "steem",
"delegatee": "tee-doc",
"vesting_shares": "9330.774703 VESTS"
}
]
}2020/11/03 04:28:36
2020/11/03 04:28:36
| delegator | steem |
| delegatee | tee-doc |
| vesting shares | 1920.017158 VESTS |
| Transaction Info | Block #48272914/Trx 0af2650bdfbcbc3a6f063da64f20d56a17affedb |
View Raw JSON Data
{
"trx_id": "0af2650bdfbcbc3a6f063da64f20d56a17affedb",
"block": 48272914,
"trx_in_block": 2,
"op_in_trx": 0,
"virtual_op": 0,
"timestamp": "2020-11-03T04:28:36",
"op": [
"delegate_vesting_shares",
{
"delegator": "steem",
"delegatee": "tee-doc",
"vesting_shares": "1920.017158 VESTS"
}
]
}2020/05/09 11:57:15
2020/05/09 11:57:15
| delegator | steem |
| delegatee | tee-doc |
| vesting shares | 9533.580062 VESTS |
| Transaction Info | Block #43224313/Trx 6c26ddaf051b12ec26d15cd923fba4028146a43f |
View Raw JSON Data
{
"trx_id": "6c26ddaf051b12ec26d15cd923fba4028146a43f",
"block": 43224313,
"trx_in_block": 8,
"op_in_trx": 0,
"virtual_op": 0,
"timestamp": "2020-05-09T11:57:15",
"op": [
"delegate_vesting_shares",
{
"delegator": "steem",
"delegatee": "tee-doc",
"vesting_shares": "9533.580062 VESTS"
}
]
}2020/05/08 16:29:06
2020/05/08 16:29:06
| delegator | steem |
| delegatee | tee-doc |
| vesting shares | 1953.311140 VESTS |
| Transaction Info | Block #43201505/Trx 4583dd86d4e05e166b3039434ee47d91890f28b1 |
View Raw JSON Data
{
"trx_id": "4583dd86d4e05e166b3039434ee47d91890f28b1",
"block": 43201505,
"trx_in_block": 22,
"op_in_trx": 0,
"virtual_op": 0,
"timestamp": "2020-05-08T16:29:06",
"op": [
"delegate_vesting_shares",
{
"delegator": "steem",
"delegatee": "tee-doc",
"vesting_shares": "1953.311140 VESTS"
}
]
}2020/01/17 20:08:03
2020/01/17 20:08:03
| delegator | steem |
| delegatee | tee-doc |
| vesting shares | 9594.314590 VESTS |
| Transaction Info | Block #40016355/Trx f80ab820efcccbddf995affbd3ccf0baebe7cebf |
View Raw JSON Data
{
"trx_id": "f80ab820efcccbddf995affbd3ccf0baebe7cebf",
"block": 40016355,
"trx_in_block": 11,
"op_in_trx": 0,
"virtual_op": 0,
"timestamp": "2020-01-17T20:08:03",
"op": [
"delegate_vesting_shares",
{
"delegator": "steem",
"delegatee": "tee-doc",
"vesting_shares": "9594.314590 VESTS"
}
]
}2019/08/22 17:42:39
2019/08/22 17:42:39
| from | dtube |
| to | tee-doc |
| amount | 0.001 STEEM |
| memo | Time is running out, claim your DTube account now before anyone else can! Login at https://d.tube |
| Transaction Info | Block #35781261/Trx 3e734ddd2951ee8b6f2cb5c160ac0945f5dae308 |
View Raw JSON Data
{
"trx_id": "3e734ddd2951ee8b6f2cb5c160ac0945f5dae308",
"block": 35781261,
"trx_in_block": 9,
"op_in_trx": 0,
"virtual_op": 0,
"timestamp": "2019-08-22T17:42:39",
"op": [
"transfer",
{
"from": "dtube",
"to": "tee-doc",
"amount": "0.001 STEEM",
"memo": "Time is running out, claim your DTube account now before anyone else can! Login at https://d.tube"
}
]
}2019/04/04 10:16:51
2019/04/04 10:16:51
| parent author | tee-doc |
| parent permlink | great-dane |
| author | steemitboard |
| permlink | steemitboard-notify-tee-doc-20190404t101650000z |
| title | |
| body | Congratulations @tee-doc! You received a personal award! <table><tr><td>https://steemitimages.com/70x70/http://steemitboard.com/@tee-doc/birthday1.png</td><td>Happy Birthday! - You are on the Steem blockchain for 1 year!</td></tr></table> <sub>_You can view [your badges on your Steem Board](https://steemitboard.com/@tee-doc) and compare to others on the [Steem Ranking](http://steemitboard.com/ranking/index.php?name=tee-doc)_</sub> ###### [Vote for @Steemitboard as a witness](https://v2.steemconnect.com/sign/account-witness-vote?witness=steemitboard&approve=1) to get one more award and increased upvotes! |
| json metadata | {"image":["https://steemitboard.com/img/notify.png"]} |
| Transaction Info | Block #31747748/Trx 04ccf5d507786c895d43505c290b8845eb05b856 |
View Raw JSON Data
{
"trx_id": "04ccf5d507786c895d43505c290b8845eb05b856",
"block": 31747748,
"trx_in_block": 5,
"op_in_trx": 0,
"virtual_op": 0,
"timestamp": "2019-04-04T10:16:51",
"op": [
"comment",
{
"parent_author": "tee-doc",
"parent_permlink": "great-dane",
"author": "steemitboard",
"permlink": "steemitboard-notify-tee-doc-20190404t101650000z",
"title": "",
"body": "Congratulations @tee-doc! You received a personal award!\n\n<table><tr><td>https://steemitimages.com/70x70/http://steemitboard.com/@tee-doc/birthday1.png</td><td>Happy Birthday! - You are on the Steem blockchain for 1 year!</td></tr></table>\n\n<sub>_You can view [your badges on your Steem Board](https://steemitboard.com/@tee-doc) and compare to others on the [Steem Ranking](http://steemitboard.com/ranking/index.php?name=tee-doc)_</sub>\n\n\n###### [Vote for @Steemitboard as a witness](https://v2.steemconnect.com/sign/account-witness-vote?witness=steemitboard&approve=1) to get one more award and increased upvotes!",
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2019/02/17 02:32:48
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2018/11/26 19:43:03
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}2018/11/18 07:49:42
2018/11/18 07:49:42
| parent author | tee-doc |
| parent permlink | re-mayowa-eu-encrybit-exchange-review-the-future-of-cryptocurrrency-exchange-20181118t001536285z |
| author | mayowa-eu |
| permlink | re-tee-doc-re-mayowa-eu-encrybit-exchange-review-the-future-of-cryptocurrrency-exchange-20181118t074933077z |
| title | |
| body | Definitely man Its gonna be the best exchange platform, just watch out! |
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2018/11/18 00:15:45
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| author | tee-doc |
| permlink | re-mayowa-eu-encrybit-exchange-review-the-future-of-cryptocurrrency-exchange-20181118t001536285z |
| title | |
| body | Nice write up, I'm sure cryptonians are gonna invest on this exchange Kudos!!! @mayowa-eu |
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}tee-docupvoted (100.00%) @mayowa-eu / encrybit-exchange-review-the-future-of-cryptocurrrency-exchange2018/11/18 00:11:33
tee-docupvoted (100.00%) @mayowa-eu / encrybit-exchange-review-the-future-of-cryptocurrrency-exchange
2018/11/18 00:11:33
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2018/08/04 13:22:27
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2018/08/04 13:16:57
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2018/07/22 19:50:54
| delegator | steem |
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tee-docupvoted (100.00%) @scottcbusiness / 9t03y7li
2018/07/07 06:58:18
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tee-docupvoted (100.00%) @scottcbusiness / 89ce9mzs
2018/07/07 06:58:18
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tee-docupvoted (100.00%) @scottcbusiness / 89ce9mzs
2018/07/07 06:58:18
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tee-docupvoted (100.00%) @scottcbusiness / philosophy-logical-fallacies-ii
2018/07/07 06:58:12
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tee-docupvoted (100.00%) @scottcbusiness / qpqssk7k
2018/07/07 06:57:48
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2018/07/07 06:57:21
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tee-docupvoted (100.00%) @scottcbusiness / n7j4vpaq
2018/07/07 06:56:42
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tee-docupvoted (100.00%) @scottcbusiness / witness-this-the-witness-report-2
2018/07/07 06:56:33
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2018/07/07 06:56:30
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tee-doccustom json: follow
2018/07/07 06:53:21
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View Raw JSON Data
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tee-doccustom json: follow
2018/07/07 06:53:06
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tee-doccustom json: follow
2018/07/07 06:52:45
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tee-doccustom json: follow
2018/07/07 06:52:39
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View Raw JSON Data
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}tee-docvoted for witness @scottcbusiness2018/07/07 06:51:12
tee-docvoted for witness @scottcbusiness
2018/07/07 06:51:12
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}2018/07/07 06:47:27
2018/07/07 06:47:27
| parent author | exyle |
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| title | |
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}tee-docupvoted (100.00%) @exyle / bye-brasil-it-was-fun-2018-07-07-06-13-212018/07/07 06:46:06
tee-docupvoted (100.00%) @exyle / bye-brasil-it-was-fun-2018-07-07-06-13-21
2018/07/07 06:46:06
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}tee-docblockchain operation: transfer to savings2018/07/07 06:43:18
tee-docblockchain operation: transfer to savings
2018/07/07 06:43:18
| from | tee-doc |
| to | tee-doc |
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View Raw JSON Data
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}2018/07/01 07:38:24
2018/07/01 07:38:24
| parent author | tee-doc |
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}tee-docfollowed @alexandered2018/06/28 22:42:33
tee-docfollowed @alexandered
2018/06/28 22:42:33
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tee-doccustom json: follow
2018/06/28 22:41:54
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tee-doccustom json: follow
2018/06/28 22:41:27
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tee-doccustom json: follow
2018/06/28 22:41:09
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tee-doccustom json: follow
2018/06/28 22:40:48
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tee-doccustom json: follow
2018/06/28 22:40:39
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tee-doccustom json: follow
2018/06/28 22:40:24
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tee-doccustom json: follow
2018/06/28 22:39:57
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tee-doccustom json: follow
2018/06/28 22:39:36
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tee-doccustom json: follow
2018/06/28 22:38:57
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}tee-docupvoted (100.00%) @uche-nna / preparation-for-representing-st-2018-5-4-21-42-522018/06/28 22:36:57
tee-docupvoted (100.00%) @uche-nna / preparation-for-representing-st-2018-5-4-21-42-52
2018/06/28 22:36:57
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2018/06/28 22:36:45
| parent author | uche-nna |
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tee-doccustom json: follow
2018/06/28 22:35:36
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}tee-docupvoted (100.00%) @tee-doc / great-dane2018/06/28 22:18:45
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}tee-docpublished a new post: safety-evaluation-of-diminazene-aceturate-on-dogs2018/06/17 11:32:39
tee-docpublished a new post: safety-evaluation-of-diminazene-aceturate-on-dogs
2018/06/17 11:32:39
| parent author | |
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| title | Safety evaluation of diminazene aceturate on dogs |
| body | EFFECT OF DIMINAZENE ACETURATE ON CANINE LEFT VENTRICULAR FUNCTIONS A PRE-DATA SEMINAR (VCJ 697) PRESENTED BY OGUNBIYI TOLUWALOPE .O MATRIC NUMBER: 20133223 DEPARTMENT OF VETERINARY PHYSIOLOGY AND PHARMACOLOGY COLLEGE OF VETERINARY MEDICINE FEDERAL UNIVERSITY OF AGRICULTURE, ABEOKUTA SUPERVISOR: DR. AJIBOLA E.S. MAY, 2018. INTRODUCTION The prevalence of Canine haemoparasitic diseases in Abeokuta and Nigeria at large is quite high (Opara et al., 2017). The treatment of these diseases is challenging and has relied heavily on the use of a wide range of different classes of anti-protozoan drugs (Irwin, 2009). Diminazene aceturate (DMZ) has been used widely by Nigerian veterinarian and has proven to be effective at the recommended dose of 3.5mg/kg (Anene et al., 2001). There are however concern about its safety. The drug is known to have a narrow safety margin and has been associated with CNS toxicity in dogs (Losos and Crockett, 1969; Donghyun et al., 2014). This toxicity is known to occur at either the normal therapeutic dose or at supra-threapeutic dose. Although direct toxicity to the heart has not been well characterized in dogs, there are reports that DMZ has a blood pressure lowering effect when administered to dogs at 3.5mg/kg (Wien 1943). The report that pentamidine, a synthetic analogue of DMZ could cause CNS and cardiac toxicity in humans Whartom et al., 1987) has further heightened the prospect of DMZ complicating the existing haemodynamic perturbations in canine patients suffering from canine babesiosis and other haemoparasitic diseases (Jacobson et al., 2000; Ajibola et al., 2016) ) Although there had been some attempts in the past to understand the mechanism of the purported hypotension caused by DMZ in dogs, there had not been a direct attempt at evaluating the cardiac functional changes that may be associated with DMZ therapy in dogs. In this current study, Echocardiography, a non-invasive technique that evaluates both cardiac structure and function (Allen, 1982; Bonagura, 1983) will be used in evaluating the haemodynamic changes associated with DMZ therapy in dogs. This technique has been used in both research and clinical practice. M-Mode echocardiography has been used in preclinical drug testing and indices obtained from this technique have been found to be reliable in cardiac function evaluation (Hanton, 2014). The aim of this study shall be to determine and compare the effects of either 3.5mg/kg and 7.0mg/kg of DMZ on cardiac function indices of dogs. The study will also correlate the changes in cardiac function indices and serum levels of DMZ in dogs administered with either of the dose. Justification The reported toxicity of diminazene aceturate to the central nervous system of dogs could cause arrhythmia in dogs by affecting the autonomic innervation to the heart. This is school of thought is further strengthened beacuse pentamidine, an anti-protozoan drug used in human and a synthetic analogue of diminazene aceturate has been reported to have pro-arrhythmic effects. The possibility of the arrhythmia causing haemodynamic disturbances is high in diminazene aceturate treated dogs. This is further worsened in dogs suffering from haemoprotozoan diseases with cardiac complications. The mechanism of the blood pressure lowering effect reported in DMZ treated dogs is not known. Since cardiac toxicity is not well characterized in dogs and there had not been a previous study evaluating the direct effect of diminazene aceturate of the heart of dogs, this study intends to use echocardiography in evaluating left ventricular functions such as cardiac-output, ejection fraction, fractional-shortening and stroke volume in dogs administered with either 3.5mg/kg or 7.0mg/kg of diminazene aceturate GENERAL OBJECTIVES To evaluate the effect of diminazene aceturate on left ventricular function of dogs Specific objectives. 1. To evaluate serial changes in the cardiac output, stroke volume, ejection fraction and 2. fractional shortening in dogs treated with either 3.5mg/kg or 7.0mg/kg 3. To correlate the changes in serum concentration of diminazene aceturate with the cardiac function indices when dogs are treated with either of the tested dosesTo compare the effects of the two doses of diminazene aceturate on the cardiac function indices studied MATERIALS AND METHODS Materials • Ten adult male dogs • 7% diminazene aceturate () • Needles and syringes • Ultrasound scanner (LEIDAL MEDICAL, England) with convex probe of frequency 5MHz. • Enzyme Linked Immunosorbent Assay test kit Experimental design The dogs will be assigned into two treatment groups comprising of five dogs each in a randomized cross-over design. Each of the dogs will be administered with either 3.5mg/kg or 7.0mg/kg of 7% Diminazene Aceturate (Hoest, England) intramuscularly. A period of 4weeks will be allowed between each treatment to allow for a complete wash-out of the drug. Echocardiography Echocardiogram will be obtained in each of the dogs using both the long and short axis para-sternal approach. Individual recordings will be made by placing the dog on the right lateral recumbency at 30minutes and just before the administration of the drug. The echocardiogram are then recorded serially at 5, 15, 30, 45, 60, and 120 minutes after the drug administration. Echocardiographic examination will be conducted with ultrasound scanner (LEIDAL MEDICAL, England) with convex probe of frequency 5MHz. The echocardiogram will be taken in standing position without anesthesia. An acoustic gel will be applied at the interface between the transducer and echo window. A 2-D guided M- mode measurement of the left ventricle at the level of papillary muscle will obtained from the short axis right parasternal view. The following indices will be obtained using the inbuilt caliper of the echocardiograph; Left ventricular internal diameter in diastole (LVIDd), Left ventricular internal diameter in systole(LVIDs), Septum wall thickness in diastole (SWTd), Septum wall thickness in systole(SWTs),Posterior wall thickness in diastole (PWTd), Posterior wall thickness in systole(PWTs), left ventricular end diastolic and systolic measurements will be taken at the largest and at the smallest dimensions between the interventricular septum and the left ventricular free wall respectively. Five individual measurement of each variable will be obtained and averaged. All measurements will be made according to standard convention. These indices will be derived according to the method of Bonagura (1983); Fractional shortening (FS) = LVIDd - LVIDs/LVIDd; Ejection Fraction (EF) = LVIDd3- LVIDs3/LVIDd3. End diastolic volume (EDV) and End systolic volume (ESV) will be derived from the formula of Teicholz (1976) as follows: EDV =7(LVIDd) 3/2.4 +LVIDd; ESV =7(LVIDs) 3/ 2.4 +LVIDs. Stroke volume (SV) in cm3 = EDV-ESV. Serum Diminazene aceturate assay The test samples would be analysed for diminazene aceturate by a competitive ELISA. Microtitre plates would be coated by placing 100 ml of the antibody optimally diluted in the coating buffer to every well. The plates would then be left at 4 0C overnight after which they would be frozen at -200C until required. When required, the plates would be thawed, washed six times with PBST(phosphate buffered saline containing Tween 20) and blotted dry. Samples would be diluted tenfold in a solution of enzyme conjugate, diluted optimally in phosphate buffered saline containing Tween 20(PBST). The diluted samples would then be loaded onto wells of antibody-coated ELISA plates,followed by overnight incubation at 4 8C. The plates would be washed as above. Colour development would be achieved by adding 100 ml of a 1:1 mixture of chromogen (TMB; Kirkengaard and Perry, Maryland USA):substrate (H2O2) per well and incubating at 370C for 10 min on a shaker incubator (Dynatech, Guernsey, Britain). The HRPO would catalyse the oxidation of TMB to a blue coloured complex. The reaction would be stopped by adding 100 ml of 1 M orthophosphoric acid per well resulting in a yellow colour. The optical densities (ODs) of the yellow solution would be measured at 450nm using a multichannel microplate reader (Bio-Tek instruments Wincoski, USA) inter-faced to a personal computer (Compaq Presario 7234, Ulsan, Korea). The drug concentrations of unknown samples would be calculated using the Eiaquik program designed by Eisler et al.(1993), from a calibration curve fitted using a fourparameter logistic regression of calibration standards. REFERENCES Ajibola, E.S, Oyewale JO, Oke, B.O (2016): Arterial blood pressure changes in acute Trypanosoma brucei infection of dogs. Bulletin of Animal Health and Production in Africa, 64(4): 73-79 Allen D.G (1982): Echocardiography as a research and clinical tool in veterinary medicine. Canadian Veterinary Journal, 23, 313-316 Anene BM., Onah, D.N., Nawa, Y (2001): Drug resistance in pathogenic African trypanosomes: What hopes for the future? Veterinary Parasitology, 96:83-100 Bonagura, J.D (1983): M- mode Echocardiography, Basic principles. Veterinary Clinics of North America: Small Animal Practice, 12:299-319 Donghyun H., WongKyoung, Y., Changbiag, H (2014): Cerebellar encephalopathy from diminazene aceturate toxicity in a dog. Korean Journal of Veterinary Research, 54(3)193-196 Eisler, M.C., Gault, E.A., Smith, H.V., Peregrine, A.S.,Holmes, P.H., 1993. Evaluation and improvement of enzyme-linked immunosorbent assay for the detection of isometamidium in bovine serum. Ther. Drug Monit. 15,236_/242 Hanton G (2014): Echocardiography, a non-invasive method for investigating preclinical drug toxicity and safety. Journal of Clinical Toxicology, 4(3); 200 Irwing P.J (2009): Canine Babesiosis; from molecular taxonomy to control. Parasitic Vectors,(suppl 1)S4 Jacobson, LS, Lobetti, RG, and Vaughan-Scott, T (2000): Blood pressure changes in Dogs with Babesiosis. Journal of South African Veterinary Association, 71(1):14-20 Losos, G.C., Crockett, E (1969): Toxicity of Berenil in the dog. Veterinary Records, 85; 196 Murilla, G.A., Eisler, M.C., Peregrine, A.S., Ndug’u, J.M.,Holmes, P.H., 1999. Development and evaluation of an enzyme-linked immunosorbent assay (ELISA) for the determination of the trypanocidal drug homidium in serum of treated cattle. J. Vet. Pharmacol. Ther. 22, 301_/307 Opara, M.N., Adewunmi, T.S., Mohammed, B.R., Obeta, S.S., Simon, MK, Jegede, OC., Agbede, RIS(2017) Investigations on the haemoprotozoan parasites of Nigerian local breeds of dogs in Gwagwalada Federal capital territory (FCT) Nigeria. Research Journal of Parasitology, 12:1-7 Teichholz L.E, Kreulen T.K, Herman M.V and Gorlin R (1976): Problems in echocardiographic volume determinations: echocardiographic angiographic correlations in the presence of absence of synergy. American Journal of Cardiology, 37: 7-11. Whartom J.M., Demopulos, PA., Goldschlager, N (1987): Torsade de pointes during administration of pentamidine Isethionate. American Journal of Medicine, 83; 571. |
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"body": "EFFECT OF DIMINAZENE ACETURATE ON CANINE LEFT VENTRICULAR FUNCTIONS\n\n\nA PRE-DATA SEMINAR\n(VCJ 697)\n\n\nPRESENTED BY\nOGUNBIYI TOLUWALOPE .O\nMATRIC NUMBER: 20133223\n\nDEPARTMENT OF VETERINARY PHYSIOLOGY AND PHARMACOLOGY\nCOLLEGE OF VETERINARY MEDICINE\nFEDERAL UNIVERSITY OF AGRICULTURE, ABEOKUTA\nSUPERVISOR: DR. AJIBOLA E.S.\n\n MAY, 2018.\nINTRODUCTION\nThe prevalence of Canine haemoparasitic diseases in Abeokuta and Nigeria at large is quite high (Opara et al., 2017). The treatment of these diseases is challenging and has relied heavily on the use of a wide range of different classes of anti-protozoan drugs (Irwin, 2009). Diminazene aceturate (DMZ) has been used widely by Nigerian veterinarian and has proven to be effective at the recommended dose of 3.5mg/kg (Anene et al., 2001). There are however concern about its safety. The drug is known to have a narrow safety margin and has been associated with CNS toxicity in dogs (Losos and Crockett, 1969; Donghyun et al., 2014). This toxicity is known to occur at either the normal therapeutic dose or at supra-threapeutic dose. Although direct toxicity to the heart has not been well characterized in dogs, there are reports that DMZ has a blood pressure lowering effect when administered to dogs at 3.5mg/kg (Wien 1943). The report that pentamidine, a synthetic analogue of DMZ could cause CNS and cardiac toxicity in humans Whartom et al., 1987) has further heightened the prospect of DMZ complicating the existing haemodynamic perturbations in canine patients suffering from canine babesiosis and other haemoparasitic diseases (Jacobson et al., 2000; Ajibola et al., 2016) )\n Although there had been some attempts in the past to understand the mechanism of the purported hypotension caused by DMZ in dogs, there had not been a direct attempt at evaluating the cardiac functional changes that may be associated with DMZ therapy in dogs. \nIn this current study, Echocardiography, a non-invasive technique that evaluates both cardiac structure and function (Allen, 1982; Bonagura, 1983) will be used in evaluating the haemodynamic changes associated with DMZ therapy in dogs. This technique has been used in both research and clinical practice. M-Mode echocardiography has been used in preclinical drug testing and indices obtained from this technique have been found to be reliable in cardiac function evaluation (Hanton, 2014). The aim of this study shall be to determine and compare the effects of either 3.5mg/kg and 7.0mg/kg of DMZ on cardiac function indices of dogs. The study will also correlate the changes in cardiac function indices and serum levels of DMZ in dogs administered with either of the dose. \nJustification \nThe reported toxicity of diminazene aceturate to the central nervous system of dogs could cause arrhythmia in dogs by affecting the autonomic innervation to the heart. This is school of thought is further strengthened beacuse pentamidine, an anti-protozoan drug used in human and a synthetic analogue of diminazene aceturate has been reported to have pro-arrhythmic effects. The possibility of the arrhythmia causing haemodynamic disturbances is high in diminazene aceturate treated dogs. This is further worsened in dogs suffering from haemoprotozoan diseases with cardiac complications. The mechanism of the blood pressure lowering effect reported in DMZ treated dogs is not known. Since cardiac toxicity is not well characterized in dogs and there had not been a previous study evaluating the direct effect of diminazene aceturate of the heart of dogs, this study intends to use echocardiography in evaluating left ventricular functions such as cardiac-output, ejection fraction, fractional-shortening and stroke volume in dogs administered with either 3.5mg/kg or 7.0mg/kg of diminazene aceturate\n\nGENERAL OBJECTIVES \nTo evaluate the effect of diminazene aceturate on left ventricular function of dogs\n\nSpecific objectives.\n1.\tTo evaluate serial changes in the cardiac output, stroke volume, ejection fraction and \n2.\tfractional shortening in dogs treated with either 3.5mg/kg or 7.0mg/kg\n3.\tTo correlate the changes in serum concentration of diminazene aceturate with the cardiac function indices when dogs are treated with either of the tested dosesTo compare the effects of the two doses of diminazene aceturate on the cardiac function indices studied \nMATERIALS AND METHODS \nMaterials\n•\tTen adult male dogs \n•\t7% diminazene aceturate ()\n•\tNeedles and syringes\n•\tUltrasound scanner (LEIDAL MEDICAL, England) with convex probe of frequency 5MHz.\n•\tEnzyme Linked Immunosorbent Assay test kit\nExperimental design\nThe dogs will be assigned into two treatment groups comprising of five dogs each in a randomized cross-over design. Each of the dogs will be administered with either 3.5mg/kg or 7.0mg/kg of 7% Diminazene Aceturate (Hoest, England) intramuscularly. A period of 4weeks will be allowed between each treatment to allow for a complete wash-out of the drug.\n\nEchocardiography\nEchocardiogram will be obtained in each of the dogs using both the long and short axis para-sternal approach. Individual recordings will be made by placing the dog on the right lateral recumbency at 30minutes and just before the administration of the drug. The echocardiogram are then recorded serially at 5, 15, 30, 45, 60, and 120 minutes after the drug administration.\nEchocardiographic examination will be conducted with ultrasound scanner (LEIDAL MEDICAL, England) with convex probe of frequency 5MHz. The echocardiogram will be taken in standing position without anesthesia. An acoustic gel will be applied at the interface between the transducer and echo window. A 2-D guided M- mode measurement of the left ventricle at the level of papillary muscle will obtained from the short axis right parasternal view. The following indices will be obtained using the inbuilt caliper of the echocardiograph; Left ventricular internal diameter in diastole (LVIDd), Left ventricular internal diameter in systole(LVIDs), Septum wall thickness in diastole (SWTd), Septum wall thickness in systole(SWTs),Posterior wall thickness in diastole (PWTd), Posterior wall thickness in systole(PWTs), left ventricular end diastolic and systolic measurements will be taken at the largest and at the smallest dimensions between the interventricular septum and the left ventricular free wall respectively. Five individual measurement of each variable will be obtained and averaged. All measurements will be made according to standard convention. These indices will be derived according to the method of Bonagura (1983); Fractional shortening (FS) = LVIDd - LVIDs/LVIDd; Ejection Fraction (EF) = LVIDd3- LVIDs3/LVIDd3.\nEnd diastolic volume (EDV) and End systolic volume (ESV) will be derived from the formula of Teicholz (1976) as follows: EDV =7(LVIDd) 3/2.4 +LVIDd; ESV =7(LVIDs) 3/ 2.4 +LVIDs. Stroke volume (SV) in cm3 = EDV-ESV. \n\nSerum Diminazene aceturate assay\nThe test samples would be analysed for diminazene aceturate by a competitive ELISA. Microtitre plates would be coated by placing 100 ml of the antibody optimally diluted in the coating buffer to every well. The plates would then be left at 4 0C overnight after which they would be frozen at -200C until required. When required, the plates would be thawed, washed six times with PBST(phosphate buffered saline containing Tween 20) and blotted dry. Samples would be diluted tenfold in a solution of enzyme conjugate, diluted optimally in phosphate buffered saline containing Tween 20(PBST). The diluted samples would then be loaded onto wells of antibody-coated ELISA plates,followed by overnight incubation at 4 8C. The plates would be washed as above. Colour development would be achieved by adding 100 ml of a 1:1 mixture of chromogen (TMB; Kirkengaard and Perry, Maryland USA):substrate (H2O2) per well and incubating at 370C for 10 min on a shaker incubator (Dynatech, Guernsey, Britain). The HRPO would catalyse the oxidation of TMB to a blue coloured complex. The reaction would be stopped by adding 100 ml of 1 M orthophosphoric acid per well resulting in a yellow colour. The optical densities (ODs) of the yellow solution would be measured at 450nm using a multichannel microplate reader (Bio-Tek instruments Wincoski, USA) inter-faced to a personal computer (Compaq Presario 7234, Ulsan, Korea). The drug concentrations of unknown samples would be calculated using the Eiaquik program designed by Eisler et al.(1993), from a calibration curve fitted using a fourparameter logistic regression of calibration standards.\n\nREFERENCES\nAjibola, E.S, Oyewale JO, Oke, B.O (2016): Arterial blood pressure changes in acute Trypanosoma brucei infection of dogs. Bulletin of Animal Health and Production in Africa, 64(4): 73-79\nAllen D.G (1982): Echocardiography as a research and clinical tool in veterinary medicine. Canadian Veterinary Journal, 23, 313-316\nAnene BM., Onah, D.N., Nawa, Y (2001): Drug resistance in pathogenic African trypanosomes: What hopes for the future? Veterinary Parasitology, 96:83-100\nBonagura, J.D (1983): M- mode Echocardiography, Basic principles. Veterinary Clinics of North America: Small Animal Practice, 12:299-319\nDonghyun H., WongKyoung, Y., Changbiag, H (2014): Cerebellar encephalopathy from diminazene aceturate toxicity in a dog. Korean Journal of Veterinary Research, 54(3)193-196\n\nEisler, M.C., Gault, E.A., Smith, H.V., Peregrine, A.S.,Holmes, P.H., 1993. Evaluation and improvement of enzyme-linked immunosorbent assay for the detection of isometamidium in bovine serum. Ther. Drug Monit. 15,236_/242\n \nHanton G (2014): Echocardiography, a non-invasive method for investigating preclinical drug toxicity and safety. Journal of Clinical Toxicology, 4(3); 200\nIrwing P.J (2009): Canine Babesiosis; from molecular taxonomy to control. Parasitic Vectors,(suppl 1)S4\nJacobson, LS, Lobetti, RG, and Vaughan-Scott, T (2000): Blood pressure changes in Dogs with Babesiosis. Journal of South African Veterinary Association, 71(1):14-20\nLosos, G.C., Crockett, E (1969): Toxicity of Berenil in the dog. Veterinary Records, 85; 196\n \nMurilla, G.A., Eisler, M.C., Peregrine, A.S., Ndug’u, J.M.,Holmes, P.H., 1999. Development and evaluation of an enzyme-linked immunosorbent assay (ELISA) for the determination of the trypanocidal drug homidium in serum of treated cattle. J. Vet. Pharmacol. Ther. 22, 301_/307\n Opara, M.N., Adewunmi, T.S., Mohammed, B.R., Obeta, S.S., Simon, MK, Jegede, OC., Agbede, RIS(2017) Investigations on the haemoprotozoan parasites of Nigerian local breeds of dogs in Gwagwalada Federal capital territory (FCT) Nigeria. Research Journal of Parasitology, 12:1-7\n\n Teichholz L.E, Kreulen T.K, Herman M.V and Gorlin R (1976): Problems in echocardiographic volume determinations: echocardiographic angiographic correlations in the presence of absence of synergy. American Journal of Cardiology, 37: 7-11.\n\n Whartom J.M., Demopulos, PA., Goldschlager, N (1987): Torsade de pointes during administration of pentamidine Isethionate. American Journal of Medicine, 83; 571.",
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}2018/06/17 11:23:27
2018/06/17 11:23:27
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