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      "memo": "Vote se-witness as one of your 30 witnesses for free votes https://steemlogin.com/sign/account-witness-vote?witness=se-witness&approve=1 |  Send 15 or more TRON to TUep3qrvP4QwqHtoXJjdQ6Q7JRQdyuaJQH with your steem username as the memo for the market value back in steem.  Or visit steemegg.com and visit the exchange tab."
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      "memo": "❗Hello lariat, You can contribute to the @roadofrich by voting as a witness. You will receive daily STEEM revenue through Witness vote. Enjoy your day 🙃"
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      "memo": "❗Hello lariat, You can contribute to the @roadofrich by voting as a witness. You will receive daily STEEM revenue through Witness vote. Enjoy your day 🙃"
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      "memo": "❗Hello lariat, You can contribute to the @roadofrich by voting as a witness. You will receive daily STEEM revenue through Witness vote. Enjoy your day 🙃"
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      "memo": "❗Hello lariat, You can contribute to the @roadofrich by voting as a witness. You will receive daily STEEM revenue through Witness vote. Enjoy your day 🙃"
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      "memo": "❗Hello lariat, You can contribute to the @roadofrich by voting as a witness. You will receive daily STEEM revenue through Witness vote. Enjoy your day 🙃"
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      "memo": "❗Hello lariat, You can contribute to the @roadofrich by voting as a witness. You will receive daily STEEM revenue through Witness vote. Enjoy your day 🙃"
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      "body": "![image.png](https://cdn.steemitimages.com/DQmR83xerpqE5k2tPUSDYrnbaa9tnGf6JqiQ2goooNTkSnN/image.png)\n\nOn June 3rd, 2018 The DASH DAO funded Medicinal Genomics to finally crack the cannabis genome. Medicinal Genomics was the first company to sequence the cannabis genome in 2011 but the sequencing technology of that era was not capable of getting the genome to the standard set by the Human Genome Project in 2001. Fast forward to 2018 and Pacific Biosciences now has tools than can sequence 20,000-100,000 base pair reads with single molecule sequencing.\n\n![image.png](https://cdn.steemitimages.com/DQma4UgK4wkYQTpC5J8gPXV66pTdWXrxd4yWfsvs5SX3fUZ/image.png)\n\nHuman Genome Project Celebration at the Whitehouse. N50 of 0.5Mb or 500Kb.\nThis major breakthrough in sequencing technology has enabled the cannabis genome to finally meet the 500kb N50 standard set by the Human Genome Project back in 2001.\n\nIn addition to the Chemdawg and LA Confidential assemblies performed in 2011 on the Illumina platform, many other parties have been contributing to public cannabis genome resources (Page, Lynch, Sawlyer, Phylos, Steep Hill) but until now no one has been able to crack the 500kb N50 barrier.\n\nThanks to DASH and the technology at Pacific Biosciences, the cannabis genome is on track to have N50s of several megabases by years end. Below is a table reflecting the various genomes sequenced to date.\n\nSeveral unique findings are already emerging. The cannabis genome is over 1 billion bases in size. This is 30% larger than most previous sequence based estimates. Additionally, we now know that CBDA Synthase and THCA Synthase are about 300kb apart and now exist on the same contig emersed in segmental duplications of other broken synthase genes. This cluster of THCA Synthase like pseudogenes is likely playing a roll in homologous recombination between CBDAS and THCAS genes. This may explain the larger centimorgan estimates of the gene distances published by Weiblen et al.\n\nThe most stunning aspect of this work is the speed at which it occurred. The grant application was submitted in mid May. The DASH master node vote came down to the last hour where (at the stroke of midnight) the proposal passed and 30 DASH were distributed to Medicinal Genomics on June 3rd. 60 days and 60 DASH later we have a genome ready for public consumption. This genome was notarized on the DASH blockchain proving its time stamp on a global public immutable ledger. A DASH a day might keep the doctor away.\n\nThe DNA assembly is currently in the polishing steps and this version will also be placed public once complete. If you would like access to the unpolished assembly please use the form at the bottom of this page.\n\nTo learn more about this project and its future sequencing efforts please visit our Crypto-Funded Public Genomics page.\n\nTo learn more about DNA assembly quality control, Adam Phillippy  delivers a great presentation about the impact of next generation sequencing read length on the quality of genomic assemblies. He also underscores the dizzying rate of improvement in sequencing technology compared to the Human Genome Project celebration in 2001. This justified Whitehouse celebration displayed an assembly quality of 500kb N50s. We only hope the Whitehouse recognizes the need for a similar celebration given the medicinal value of Cannabis expressed in various Department of Health and Human Services patents.\n\n![image.png](https://cdn.steemitimages.com/DQmZQ9JhHGsyMhvwT8yeAyzysQmoNA7nJgKGRMwLjbVHdYc/image.png)\n\nDownload the Jamaican Lion DNA Assembly File\nThe DNA assembly is currently in the polishing steps and will be placed public once complete. If you would like access to the unpolished Assembly please complete the form below and we will email a link to the file.\n\nhttps://www.medicinalgenomics.com/dash-powered-genomics/\nhttps://www.medicinalgenomics.com/crypto-funded-public-genomics/",
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