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      "permlink": "phages-against-american-foulbrood",
      "title": "Phages against American Foulbrood?",
      "body": "@@ -34,24 +34,30 @@\n millions of \n+honey \n bees face on\n@@ -172,22 +172,16 @@\n  in the \n-whole \n hive. It\n@@ -1036,76 +1036,9 @@\n ia %3C\n-a href=%22https://en.wikipedia.org/wiki/Paenibacillus%22 target=%22_blank%22\n+i\n %3EPae\n@@ -1072,11 +1072,15 @@\n ae%3C/\n-a\n+i\n %3E.\n+ %5B1%5D\n %3Cbr%3E\n@@ -1557,16 +1557,105 @@\n ood food\n+, also know as %3Ca href=%22https://drapis.com/#bread%22 target=%22_blank%22%3Ebee bread or perga%3C/a%3E\n . Thus p\n@@ -1854,16 +1854,20 @@\n  entire \n+bee \n hive bec\n@@ -2197,58 +2197,11 @@\n es. \n-- https://en.wikipedia.org/wiki/American_foulbrood\n+%5B2%5D\n %3Cbr%3E\n@@ -2454,17 +2454,53 @@\n nt. \n-Therefore\n+In order to prevent the loss of more colonies\n , be\n@@ -2572,16 +2572,20 @@\n t about \n+bee \n %3Ca href=\n@@ -3025,114 +3025,19 @@\n ral \n- %3Ca href=%22https://www.sciencedirect.com/science/article/pii/S0378113508001466%22 target=%22_blank%22%3E\n studies\n-%3C/a%3E\n+ %5B3%5D\n  sho\n@@ -3042,24 +3042,28 @@\n how us that \n+bee \n propolis has\n@@ -3106,21 +3106,16 @@\n r, they \n-also \n demonstr\n@@ -3127,22 +3127,58 @@\n hat \n-propolis is no\n+although propolis benefits colonies, it still isn'\n t a \n@@ -3191,19 +3191,19 @@\n y itself\n-, b\n+. B\n ut rathe\n@@ -3326,24 +3326,83 @@\n hat the \n-propolis\n+%3Ca href=%22https://drapis.com/#propolis%22 target=%22_blank%22%3Epropolis%3C/a%3E\n  envelop\n@@ -3807,277 +3807,11 @@\n FB. \n-Check %3Ca href=%22https://www.beelab.umn.edu/sites/beelab.umn.edu/files/borba_et_al-2017-scientific_reports.pdf%22 target=%22_blank%22%3EPropolis envelope in %3Ci%3EApis mellifera%3C/i%3E colonies supports honey bees against the pathogen, %3Ci%3EPaenibacillus larvae%3C/i%3E%3C/a%3E for more details.\n+%5B4%5D\n %3Cbr%3E\n@@ -4347,211 +4347,11 @@\n e.%E2%80%9D \n-- %3Ca href=%22https://news.byu.edu/news/using-microscopic-bugs-save-bees?fbclid=IwAR3C27rSO_9_LesbTS7lV9yTQoogpn8qnY4_C_d4TubW5_gowlR-hUm38-Y%3C/p%3E%22 target=%22_blank%22%3EUsing microscopic bugs to save the bees%3C/a%3E\n+%5B5%5D\n %0A%0A%3Cp\n@@ -5888,16 +5888,20 @@\n ulbrood.\n+ %5B6%5D\n %3Cbr%3E%0A%3Cbr\n@@ -5906,34 +5906,912 @@\n br%3E%0A\n-For further reading check \n+%3Ch4%3EReferences:%3C/h4%3E%0A%3Ca href=%22https://en.wikipedia.org/wiki/Paenibacillus%22 target=%22_blank%22%3E%5B1%5D%3C/a%3E%3Ci%3EPaenibacillus larvae ssp. larvae%3C/i%3E%3Cbr%3E%0A%3Ca href=%22https://en.wikipedia.org/wiki/American_foulbrood%22 target=%22_blank%22%3E%5B2%5D%3C/a%3E American Foulbrood - Wikipedia%3Cbr%3E%0A%3Ca href=%22https://www.sciencedirect.com/science/article/pii/S0378113508001466%22 target=%22_blank%22%3E%5B3%5D%3C/a%3E Efficacy of natural propolis extract in the control of American Foulbrood%3Cbr%3E%0A%3Ca href=%22https://www.beelab.umn.edu/sites/beelab.umn.edu/files/borba_et_al-2017-scientific_reports.pdf%22 target=%22_blank%22%3E%5B4%5D%3C/a%3E Propolis envelope in %3Ci%3EApis mellifera%3C/i%3E colonies supports honey bees against the pathogen, %3Ci%3EPaenibacillus larvae%3C/i%3E%3Cbr%3E%0A%3Ca href=%22https://news.byu.edu/news/using-microscopic-bugs-save-bees?fbclid=IwAR3C27rSO_9_LesbTS7lV9yTQoogpn8qnY4_C_d4TubW5_gowlR-hUm38-Y%3C/p%3E%22 target=%22_blank%22%3E%5B5%5D%3C/a%3E Using microscopic bugs to save the bees%3Cbr%3E%0A\n %3Ca h\n@@ -6904,16 +6904,24 @@\n _blank%22%3E\n+%5B6%5D%3C/a%3E \n Characte\n@@ -6932,16 +6932,19 @@\n tion of \n+%3Ci%3E\n Paenibac\n@@ -6955,16 +6955,20 @@\n s larvae\n+%3C/i%3E\n  bacteri\n@@ -7035,18 +7035,18 @@\n ophages%3C\n-/a\n+br\n %3E%0A%3C/p%3E%0A%0A\n",
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      "permlink": "phages-against-american-foulbrood",
      "title": "Phages against American Foulbrood?",
      "body": "@@ -732,24 +732,30 @@\n n Fouldbrood\n+ (AFB)\n .%3Cbr%3E%0A%3Cbr%3E%0A%0A\n@@ -753,17 +753,216 @@\n r%3E%0A%3Cbr%3E%0A\n-%0A\n+The nests of densely populated social insect colonies provide a favorable habitat for a wide range of parasites %0Aand pathogens that have evolved to overwhelm or suppress their hosts%E2%80%99 immune defenses. \n AFB is f\n@@ -2505,34 +2505,1556 @@\n can \n-foulbrood should be burned\n+Foulbrood should be burned.%3Cbr%3E%0A%3Chr%3E%0A%3Ch4%3EWhat about %3Ca href=%22https://drapis.com/#propolis%22 target=%22_blank%22%3EPropolis%3C/a%3E?%3C/h4%3E%0AHoney bees have immune defenses both as individuals and as a colony (e.g., individual and social %0Aimmunity). One form of honey bee social immunity is the %3Ca href=%22https://drapis.com/#propolis%22 target=%22_blank%22%3Ecollection of antimicrobial plant resins%3C/a%3E and the %0Adeposition of the resins as a propolis envelope within the nest. Thus working as a natural defense. Several  %3Ca href=%22https://www.sciencedirect.com/science/article/pii/S0378113508001466%22 target=%22_blank%22%3Estudies%3C/a%3E show us that propolis has a significant impact on AFB. However, they also demonstrate that propolis is not a cure by itself, but rather a prevention method, since it doesn't completely eradicate the spore presence in the hive.%3Cbr%3E%0A%3Cbr%3E%0AStudies show that the propolis envelope serves as an antimicrobial layer around the colony protecting the brood from bacterial pathogen infection, resulting in a lower colony-level infection load. Antimicrobial activity of larval food was significantly higher when challenged colonies had a propolis envelope compared to colonies without the envelope. In addition, colonies with a propolis envelope had significantly reduced levels of AFB. Check %3Ca href=%22https://www.beelab.umn.edu/sites/beelab.umn.edu/files/borba_et_al-2017-scientific_reports.pdf%22 target=%22_blank%22%3EPropolis envelope in %3Ci%3EApis mellifera%3C/i%3E colonies supports honey bees against the pathogen, %3Ci%3EPaenibacillus larvae%3C/i%3E%3C/a%3E for more details\n .%3Cbr\n",
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      "body": "<p>Year after year, millions and millions of bees face one of their biggest enemies. One that eats them from inside out. It targets the youngest and most defenseless in the whole hive. It lasts until the whole colony is subdued.</p>\n\n<center>https://cdn.steemitimages.com/DQmQBpES5VicqefTvZHnDvNx62v9rPMreDHqFsuf7jg8wA3/american%20foulbrood.jpg<br>\n<p>American Foulbrood Symptoms: Ropey Dead Larvae and Black Scale.<br>Photo credit: Michael E Wilson</p></center><br>\n\n<p>Pick a cell with sunken, perforated cappings. When pulling the stick out from infected brood, a brown, ropey slime can be seen. Also pictured here are many cells with black, bacterial spore laden scales on the bottom of the cells, a condition unique to American Fouldbrood.<br>\n<br>\n\nAFB is found almost worldwide, and it is caused by the spore-forming bacteria <a href=\"https://en.wikipedia.org/wiki/Paenibacillus\" target=\"_blank\">Paenibacillus larvae ssp. larvae</a>.<br>\n<br>\nLarvae up to 3 days old become infected by ingesting spores that are present in their food. Young larvae less than 24 hours old are most susceptible to infection. Spores germinate in the gut of the larva, where the vegetative form of the bacteria begins to grow, feeding from the larva. Infected larvae darken and die, normally after their cell is sealed.<br>\n<br>\nWhen cleaning infected cells, bees distribute spores throughout the entire colony, contaminating brood food. Thus perpetuating the cycle. Nectar stored in contaminated cells will contain spores and soon the brood chamber becomes filled with contaminated honey. As this honey is moved up into the supers, the entire hive becomes contaminated with spores. When the colony becomes weak from AFB infection, robber bees may enter and take contaminated honey back to their hives thereby spreading the disease to other colonies and apiaries. Beekeepers also may spread disease by moving equipment (frames or supers) from contaminated hives to healthy ones. - https://en.wikipedia.org/wiki/American_foulbrood<br>\n<br>\nTreating Foulbrood is complicated because the disease can evolve to resist antibiotics and other chemical treatments. Spores are extremely resistant to desiccation and can remain viable for more than 40 years in honey and beekeeping equipment. Therefore, beehives with American foulbrood should be burned.<br>\n<hr>\n<div class=\"pull-left\"><center>https://steemitimages.com/165x165/https://cdn.steemitimages.com/DQmcLQyz9qeRzUumuvys7BLoun2K6F3m2ZvSMuQvg5t3w3e/491px-PhageExterior.svg.png<br>\n<p>Phage</p></center></div>\n\"Phages are the most abundant life form on the planet and each phage has a unique bacteria that it will attack,” said Sandra Burnett, BYU professor of microbiology and molecular biology. “This makes phage an ideal treatment for bacterial disease because it can target specific bacteria while leaving all other cells alone.” - <a href=\"https://news.byu.edu/news/using-microscopic-bugs-save-bees?fbclid=IwAR3C27rSO_9_LesbTS7lV9yTQoogpn8qnY4_C_d4TubW5_gowlR-hUm38-Y</p>\" target=\"_blank\">Using microscopic bugs to save the bees</a>\n\n<p><center><h4>But could these lunar lander like virus be the solution to one of bees' biggest enemies? Phages against the American Foulbrood.</h4></center></p>\n<br>\n<div class=\"pull-right\"><center><img src=\"https://steemitimages.com/450x450/https://cdn.steemitimages.com/DQmWkRERJQnRQcbEhRAcjhSH7hrqV669DJK8rMYH3QX5WXF/lunar%20lander.jpg\"><br>\n<p>Lunar Lander</p></center></div>\n\n<p>\"A bacteriophage, AKA a phage, is a virus that infects and replicates within Bacteria and Archaea. The term was derived from \"bacteria\" and the Greek \"to devour\". Phages replicate within the bacterium following the injection of their genome into its cytoplasm.<br>\n<br>Bacteriophages are among the most common and diverse entities in the biosphere. Ubiquitous viruses, found wherever bacteria exist. It is estimated there are more than 1031 bacteriophages on the planet, more than every other organism on Earth, including bacteria, combined. - https://en.wikipedia.org/wiki/Bacteriophage</p>\n\n<p>Brigham Young University researchers have identified five new phages that can potentially treat honeybee hives infected with American Foulbrood.</p>\n\nhttps://youtu.be/rj9_QGBJN0w\n<br>\n<p>Using phage against American Foulbrood is an organic treatment, that uses nature's natural bacteria killers. Definitely preferable to antibiotics, chemical and pesticide use that otherwise would be present in honey and all other bee products. This analysis provides a foundation on which to build an understanding of the natural predators of the disease American Foulbrood.<br>\n<br>\nFor further reading check <a href=\"https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-15-745\" target=\"_blank\">Characterization of Paenibacillus larvae bacteriophages and their genomic relationships to firmicute bacteriophages</a>\n</p>\n\n<hr>\n<center>Premium raw apiculture products from Alentejo, Portugal<br><a href=\"https://drapis.com/#honey\" target=\"_blank\">Raw Honey</a>, <a href=\"https://drapis.com/#comb\" target=\"_blank\">Comb Honey</a>, <a href=\"https://drapis.com/#wax\" target=\"_blank\">Beeswax</a>, <a href=\"https://drapis.com/#propolis\" target=\"_blank\">Propolis</a> and <a href=\"https://drapis.com/#bread\" target=\"_blank\">Bee Bread</a><br>Worldwide shipping</center>\n<hr>\n<center>\n<a href=\"http://www.drapis.com\" target=\"_blank\"><img src=\"https://steemitimages.com/640x0/https://cdn.steemitimages.com/DQmf4ZpqnHWqiZ91CCjDVMMB46c5nZPFgVL7EXQwJ7rU6LM/drapis-logo.png\" alt=\"drapis-logo.png\"><br>\ndrapis.com</a><br>\n<a href=\"/@drapis\">@drapis</a><br>\n<hr>\n<p><a href=\"https://steemit.com/introduceyourself/@drapis/our-dream\" target=\"_blank\">Traditional, sustainable beekeeping. Stubborn resistance to chemicals or pesticides.<br>\nSuccess measured by number of healthy colonies, not by honey Kg.</a></p>\n<hr>\nTwitter <a href=\"https:// https://twitter.com/drapiscom\" target=\"_blank\">@drapiscom</a> // \nFacebook <a href=\"https://www.facebook.com/drapiscom/\" target=\"_blank\">@drapiscom</a> // \nInstagram <a href=\"https://www.instagram.com/dr.apis/\" target=\"_blank\">@dr.apis</a>\n</center>",
      "json_metadata": "{\"tags\":[\"science\",\"nature\",\"food\",\"news\",\"health\"],\"image\":[\"https://cdn.steemitimages.com/DQmQBpES5VicqefTvZHnDvNx62v9rPMreDHqFsuf7jg8wA3/american%20foulbrood.jpg\",\"https://steemitimages.com/165x165/https://cdn.steemitimages.com/DQmcLQyz9qeRzUumuvys7BLoun2K6F3m2ZvSMuQvg5t3w3e/491px-PhageExterior.svg.png\",\"https://steemitimages.com/450x450/https://cdn.steemitimages.com/DQmWkRERJQnRQcbEhRAcjhSH7hrqV669DJK8rMYH3QX5WXF/lunar%20lander.jpg\",\"https://img.youtube.com/vi/rj9_QGBJN0w/0.jpg\",\"https://steemitimages.com/640x0/https://cdn.steemitimages.com/DQmf4ZpqnHWqiZ91CCjDVMMB46c5nZPFgVL7EXQwJ7rU6LM/drapis-logo.png\"],\"links\":[\"https://en.wikipedia.org/wiki/Paenibacillus\",\"https://en.wikipedia.org/wiki/American_foulbrood\",\"https://news.byu.edu/news/using-microscopic-bugs-save-bees?fbclid=IwAR3C27rSO_9_LesbTS7lV9yTQoogpn8qnY4_C_d4TubW5_gowlR-hUm38-Y</p>\",\"https://en.wikipedia.org/wiki/Bacteriophage\",\"https://youtu.be/rj9_QGBJN0w\",\"https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-15-745\",\"https://drapis.com/#honey\",\"https://drapis.com/#comb\",\"https://drapis.com/#wax\",\"https://drapis.com/#propolis\",\"https://drapis.com/#bread\",\"http://www.drapis.com\",\"/@drapis\",\"https://steemit.com/introduceyourself/@drapis/our-dream\",\"https:// https://twitter.com/drapiscom\",\"https://www.facebook.com/drapiscom/\",\"https://www.instagram.com/dr.apis/\"],\"app\":\"steemit/0.1\",\"format\":\"markdown\"}"
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      "permlink": "re-drapis-re-joeyarnoldvn-re-drapis-dear-dr-apis-friend-20181216t214205023z",
      "title": "",
      "body": "Awesome.",
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    {
      "parent_author": "sifondeseltz",
      "parent_permlink": "re-drapis-dear-dr-apis-friend-20181215t113033809z",
      "author": "drapis",
      "permlink": "re-sifondeseltz-re-drapis-dear-dr-apis-friend-20181215t155436777z",
      "title": "",
      "body": "@@ -514,16 +514,17 @@\n  to lear\n+n\n  a bit m\n",
      "json_metadata": "{\"tags\":[\"introduceyourself\"],\"users\":[\"sifondeseltz\"],\"links\":[\"www.drapis.com\",\"https://steemit.com/introduceyourself/@drapis/our-dream\"],\"app\":\"steemit/0.1\"}"
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      "author": "drapis",
      "permlink": "re-joeyarnoldvn-re-drapis-dear-dr-apis-friend-20181215t154604922z",
      "title": "",
      "body": "@@ -605,16 +605,16 @@\n ng appro\n-u\n+a\n ch%3C/a%3E.\n",
      "json_metadata": "{\"tags\":[\"introduceyourself\"],\"users\":[\"joeyarnoldvn\"],\"links\":[\"www.drapis.com\",\"https://www.youtube.com/watch?v=M3ZVzH9H0wA\",\"https://steemit.com/introduceyourself/@drapis/our-dream\"],\"app\":\"steemit/0.1\"}"
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      "parent_permlink": "introduceyourself",
      "author": "drapis",
      "permlink": "dear-dr-apis-friend",
      "title": "Dear Dr Apis friend",
      "body": "@@ -614,32 +614,53 @@\n %0A%0A%3Ccenter%3E!%5B\n-all1.jpg\n+Dr Apis Raw Unprocessed Honey\n %5D(https://cd\n@@ -1341,24 +1341,50 @@\n enter%3E!%5B\n-all1.jpg\n+Dr Apis Raw Unprocessed Comb Honey\n %5D(https:\n@@ -1472,24 +1472,35 @@\n %0A%3Cbr%3E%0A!%5B\n-all2.jpg\n+Dr Apis Raw Bee Wax\n %5D(https:\n@@ -1889,27 +1889,41 @@\n enter%3E!%5B\n-group01.jpg\n+Dr Apis Propolis Tincture\n %5D(https:\n@@ -3675,32 +3675,49 @@\n enter%3E!%5B\n-beebread_big.jpg\n+Dr Apis Raw Unprocessed Bee Bread\n %5D(https:\n",
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      "parent_author": "drapis",
      "parent_permlink": "dear-dr-apis-friend",
      "author": "steemitboard",
      "permlink": "steemitboard-notify-drapis-20181215t171005000z",
      "title": "",
      "body": "Congratulations @drapis! You have completed the following achievement on the Steem blockchain and have been rewarded with new badge(s) :\n\n<table><tr><td>https://steemitimages.com/60x70/http://steemitboard.com/@drapis/comments.png?201812151619</td><td>You made more than 10 comments. Your next target is to reach 50 comments.</td></tr>\n</table>\n\n<sub>_[Click here to view your Board of Honor](https://steemitboard.com/@drapis)_</sub>\n<sub>_If you no longer want to receive notifications, reply to this comment with the word_ `STOP`</sub>\n\n\n\n> Support [SteemitBoard's project](https://steemit.com/@steemitboard)! **[Vote for its witness](https://v2.steemconnect.com/sign/account-witness-vote?witness=steemitboard&approve=1)** and **get one more award**!",
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  "op": [
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      "parent_author": "steemflow",
      "parent_permlink": "steemflow-re-drapis-our-dream-20181210t033113152z",
      "author": "drapis",
      "permlink": "re-steemflow-steemflow-re-drapis-our-dream-20181215t160116653z",
      "title": "",
      "body": "@@ -55,8 +55,115 @@\n mmunity!\n+ I would love to engage and contribute in community development. Where are the discussion boards? Thank you\n",
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      "author": "drapis",
      "permlink": "re-steemflow-steemflow-re-drapis-our-dream-20181215t160116653z",
      "title": "",
      "body": "Hi @steemflow! Thank you, we are happy to join this community!",
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      "author": "drapis",
      "permlink": "re-uday20-re-drapis-our-dream-20181215t155712252z",
      "title": "",
      "body": "Hello @uday20 thank you!",
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      "author": "drapis",
      "permlink": "re-bolus-bolus-re-drapis-our-dream-20181210t174634477z",
      "title": "",
      "body": "Hej @bolus! How is this @partiko app working out for you?",
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      "parent_permlink": "re-drapis-dear-dr-apis-friend-20181215t113033809z",
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      "permlink": "re-sifondeseltz-re-drapis-dear-dr-apis-friend-20181215t155436777z",
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      "body": "Hi @sifondeseltz <3 absolutely. And as you can imagine, packaging is something we look into with great detail. Not only from an environmental perspective, but also for the inherent risks of international shipping. Did you know that most of <a href=\"www.drapis.com\" target=\"_blank\">Dr Apis</a> packaging consists in reused packaging materials that would otherwise be wasted? I invite you to check <a href=\"https://steemit.com/introduceyourself/@drapis/our-dream\" target=\"_blank\">Our Dream</a> post, here on Steemit to lear a bit more about <a href=\"www.drapis.com\" target=\"_blank\">Dr Apis</a>.",
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      "permlink": "re-joeyarnoldvn-re-drapis-dear-dr-apis-friend-20181215t154604922z",
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      "body": "Hi @joeyarnoldvn <3 you bet! And the more we work, the more we fall in love with <a href=\"www.drapis.com\" target=\"_blank\">Dr Apis</a>. The intense flavors and sheer quality of our bees' work is sublime. For sure there are many other quality producers worldwide but then, this is like wine... Each region has it's unique qualities. And here we are. <a href=\"https://www.youtube.com/watch?v=M3ZVzH9H0wA\" target=\"_blank\">Alentejo</a>, Portugal at it's best! Here you can learn more about our <a href=\"https://steemit.com/introduceyourself/@drapis/our-dream\" target=\"_blank\">minimalistic sustainable beekeeping approuch</a>.",
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      "body": "I love to eat everything and if it is well packed it tastes even better.Welcome to Steemit.",
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      "author": "steemitboard",
      "permlink": "steemitboard-notify-drapis-20181214t201329000z",
      "title": "",
      "body": "Congratulations @drapis! You have completed the following achievement on the Steem blockchain and have been rewarded with new badge(s) :\n\n<table><tr><td>https://steemitimages.com/60x60/http://steemitboard.com/notifications/firstcommented.png</td><td>You got a First Reply</td></tr>\n</table>\n\n<sub>_[Click here to view your Board of Honor](https://steemitboard.com/@drapis)_</sub>\n<sub>_If you no longer want to receive notifications, reply to this comment with the word_ `STOP`</sub>\n\n\n\n> Support [SteemitBoard's project](https://steemit.com/@steemitboard)! **[Vote for its witness](https://v2.steemconnect.com/sign/account-witness-vote?witness=steemitboard&approve=1)** and **get one more award**!",
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      "parent_author": "drapis",
      "parent_permlink": "re-homesteaderscoop-re-nealmcspadden-my-honey-came-in-20181214t145039872z",
      "author": "homesteaderscoop",
      "permlink": "re-drapis-re-homesteaderscoop-re-nealmcspadden-my-honey-came-in-20181214t183451812z",
      "title": "",
      "body": "Hello @drapis :) Yes we are open to vendors and customers worldwide. It may not be obvious yet but we have vendors in over 5 countries already. Your website store looks very beautiful. How large is your company? our goal is in supporting individuals and mom & pop size businesses.",
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      "body": "I love honey, honey.",
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